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antibody against cd13  (Bio-Rad)


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    Bio-Rad antibody against cd13
    Antibody Against Cd13, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibody+against+cd13/Rat+anti+Mouse+CD13/pm40066845-108-10-13
    Average 93 stars, based on 93 article reviews
    antibody against cd13 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Longitudinal Investigation of Brain and Spinal Cord Pericytes After Inducible PDGFRβ + Cell Ablation in Adult Mice.
    Article Snippet: For immunofluorescence, sections underwent blocking and permeabilization with a solution containing 5% normal goat serum (NGS) (Sigma- Aldrich, Cat. no. G9023), 2% bovine serum albumin (BSA) (Cat. No. A4919, CAS No. 9048- 46- 8), and 0.1% Triton X- 100 (Sigma- Aldrich, Cat. No. 648466, CAS No. CAS_9002- 93- 1) in Dulbecco's phosphatebuffered saline (DPBS) (Sigma- Aldrich, Cat. No. D8537) for 1 h at room temperature. .. This was followed by overnight incubation at 4°C with primary antibody against CD13 (Bio- Rad, Cat. No. MCA2183) at a 1:100 dilution in the blocking solution. .. After primary antibody incubation, sections were rinsed in DPBS and then incubated with Alexa Fluor 647- labeled anti- rabbit IgG (Abcam, Cat. No. ab150159) and DyLight 488–conjugated tomato lectin (Vector Laboratories, Cat. No. DL- 1174), at 1:200 dilution for 2 h at room temperature to visualize pericytes and vasculature, respectively.

    Article Title: Longitudinal Investigation of Brain and Spinal Cord Pericytes After Inducible PDGFRβ + Cell Ablation in Adult Mice
    Article Snippet: For immunofluorescence, sections underwent blocking and permeabilization with a solution containing 5% normal goat serum (NGS) (Sigma‐Aldrich, Cat. no. G9023), 2% bovine serum albumin (BSA) (Cat. No. A4919, CAS No. 9048‐46‐8), and 0.1% Triton X‐100 (Sigma‐Aldrich, Cat. No. 648466, CAS No. CAS_9002‐93‐1) in Dulbecco's phosphate‐buffered saline (DPBS) (Sigma‐Aldrich, Cat. No. D8537) for 1 h at room temperature. .. This was followed by overnight incubation at 4°C with primary antibody against CD13 (Bio‐Rad, Cat. No. MCA2183) at a 1:100 dilution in the blocking solution. .. After primary antibody incubation, sections were rinsed in DPBS and then incubated with Alexa Fluor 647‐labeled anti‐rabbit IgG (Abcam, Cat. No. ab150159) and DyLight 488–conjugated tomato lectin (Vector Laboratories, Cat. No. DL‐1174), at 1:200 dilution for 2 h at room temperature to visualize pericytes and vasculature, respectively.

    Article Title: A Novel Mouse Model Demonstrates In Vivo Replenishment of Central Nervous System Pericytes After Successful Acute Ablation
    Article Snippet: .. This was followed by overnight incubation at 4°C with primary antibody against CD13 (Bio-Rad, MCA2183) at a 1:100 dilution in the blocking solution. .. After primary antibody incubation, sections were rinsed in DPBS and then incubated with Alexa Fluor 647-labeled anti-rabbit IgG (Abcam, ab150159) and Dylight 488-conjugated tomato lectin (Vector Laboratories, DL-1174), at 1:200 dilution for 2 hours at room temperature to visualize pericytes and vasculature, respectively.

    Blocking Assay:

    Article Title: Longitudinal Investigation of Brain and Spinal Cord Pericytes After Inducible PDGFRβ + Cell Ablation in Adult Mice.
    Article Snippet: For immunofluorescence, sections underwent blocking and permeabilization with a solution containing 5% normal goat serum (NGS) (Sigma- Aldrich, Cat. no. G9023), 2% bovine serum albumin (BSA) (Cat. No. A4919, CAS No. 9048- 46- 8), and 0.1% Triton X- 100 (Sigma- Aldrich, Cat. No. 648466, CAS No. CAS_9002- 93- 1) in Dulbecco's phosphatebuffered saline (DPBS) (Sigma- Aldrich, Cat. No. D8537) for 1 h at room temperature. .. This was followed by overnight incubation at 4°C with primary antibody against CD13 (Bio- Rad, Cat. No. MCA2183) at a 1:100 dilution in the blocking solution. .. After primary antibody incubation, sections were rinsed in DPBS and then incubated with Alexa Fluor 647- labeled anti- rabbit IgG (Abcam, Cat. No. ab150159) and DyLight 488–conjugated tomato lectin (Vector Laboratories, Cat. No. DL- 1174), at 1:200 dilution for 2 h at room temperature to visualize pericytes and vasculature, respectively.

    Article Title: Longitudinal Investigation of Brain and Spinal Cord Pericytes After Inducible PDGFRβ + Cell Ablation in Adult Mice
    Article Snippet: For immunofluorescence, sections underwent blocking and permeabilization with a solution containing 5% normal goat serum (NGS) (Sigma‐Aldrich, Cat. no. G9023), 2% bovine serum albumin (BSA) (Cat. No. A4919, CAS No. 9048‐46‐8), and 0.1% Triton X‐100 (Sigma‐Aldrich, Cat. No. 648466, CAS No. CAS_9002‐93‐1) in Dulbecco's phosphate‐buffered saline (DPBS) (Sigma‐Aldrich, Cat. No. D8537) for 1 h at room temperature. .. This was followed by overnight incubation at 4°C with primary antibody against CD13 (Bio‐Rad, Cat. No. MCA2183) at a 1:100 dilution in the blocking solution. .. After primary antibody incubation, sections were rinsed in DPBS and then incubated with Alexa Fluor 647‐labeled anti‐rabbit IgG (Abcam, Cat. No. ab150159) and DyLight 488–conjugated tomato lectin (Vector Laboratories, Cat. No. DL‐1174), at 1:200 dilution for 2 h at room temperature to visualize pericytes and vasculature, respectively.

    Article Title: A Novel Mouse Model Demonstrates In Vivo Replenishment of Central Nervous System Pericytes After Successful Acute Ablation
    Article Snippet: .. This was followed by overnight incubation at 4°C with primary antibody against CD13 (Bio-Rad, MCA2183) at a 1:100 dilution in the blocking solution. .. After primary antibody incubation, sections were rinsed in DPBS and then incubated with Alexa Fluor 647-labeled anti-rabbit IgG (Abcam, ab150159) and Dylight 488-conjugated tomato lectin (Vector Laboratories, DL-1174), at 1:200 dilution for 2 hours at room temperature to visualize pericytes and vasculature, respectively.



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    Brain pericytes increase glutamate uptake in astrocyte cultures. (A) Double immunofluorescence staining for <t>CD13/EAAT1</t> (top left panel), CD13/EAAT2 (top right panel), GFAP/EAAT1 (bottom left panel), and GFAP/EAAT2 (bottom right panel) in intact mouse hippocampus sections. CD13 and GFAP signals appear green, while EAAT1 and EAAT2 signals appear red. (B) Regression analyses of cell/medium ratios of [ 3 H]-L-glutamate ([ 3 H]-L-Glu) uptake versus incubation time in astrocytes cultured without pericytes (astrocyte monocultures) or with pericytes (pericyte co-cultures). Astrocytes in monocultures and pericyte co-cultures were incubated in uptake buffer containing [ 3 H]-L-Glu for 1, 2, 5, and 10 min. A linear regression analysis was also performed to obtain the rate of [ 3 H]-L-Glu uptake. The slope of the lines was 9.48 in astrocyte monocultures ( r 2 = 0.90) and 13.54 in pericyte co-cultures ( r 2 = 0.89). Values are expressed as means ± SEM (n = 4 per point). * p < 0.05, between astrocyte monocultures and pericyte co-cultures.
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    Brain pericytes increase glutamate uptake in astrocyte cultures. (A) Double immunofluorescence staining for <t>CD13/EAAT1</t> (top left panel), CD13/EAAT2 (top right panel), GFAP/EAAT1 (bottom left panel), and GFAP/EAAT2 (bottom right panel) in intact mouse hippocampus sections. CD13 and GFAP signals appear green, while EAAT1 and EAAT2 signals appear red. (B) Regression analyses of cell/medium ratios of [ 3 H]-L-glutamate ([ 3 H]-L-Glu) uptake versus incubation time in astrocytes cultured without pericytes (astrocyte monocultures) or with pericytes (pericyte co-cultures). Astrocytes in monocultures and pericyte co-cultures were incubated in uptake buffer containing [ 3 H]-L-Glu for 1, 2, 5, and 10 min. A linear regression analysis was also performed to obtain the rate of [ 3 H]-L-Glu uptake. The slope of the lines was 9.48 in astrocyte monocultures ( r 2 = 0.90) and 13.54 in pericyte co-cultures ( r 2 = 0.89). Values are expressed as means ± SEM (n = 4 per point). * p < 0.05, between astrocyte monocultures and pericyte co-cultures.
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    Brain pericytes increase glutamate uptake in astrocyte cultures. (A) Double immunofluorescence staining for <t>CD13/EAAT1</t> (top left panel), CD13/EAAT2 (top right panel), GFAP/EAAT1 (bottom left panel), and GFAP/EAAT2 (bottom right panel) in intact mouse hippocampus sections. CD13 and GFAP signals appear green, while EAAT1 and EAAT2 signals appear red. (B) Regression analyses of cell/medium ratios of [ 3 H]-L-glutamate ([ 3 H]-L-Glu) uptake versus incubation time in astrocytes cultured without pericytes (astrocyte monocultures) or with pericytes (pericyte co-cultures). Astrocytes in monocultures and pericyte co-cultures were incubated in uptake buffer containing [ 3 H]-L-Glu for 1, 2, 5, and 10 min. A linear regression analysis was also performed to obtain the rate of [ 3 H]-L-Glu uptake. The slope of the lines was 9.48 in astrocyte monocultures ( r 2 = 0.90) and 13.54 in pericyte co-cultures ( r 2 = 0.89). Values are expressed as means ± SEM (n = 4 per point). * p < 0.05, between astrocyte monocultures and pericyte co-cultures.
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    antibodies against cd90, cd73, cd13, cd45, hla-dr  (Becton Dickinson)
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    Human amnion-derived mesenchymal stromal/stem cells (hAMSCs) were grown as monolayers with or without priming. (A) Representative DIC images of hAMSCs grown as monolayers without priming (hAMSCs), cultured under hypoxic conditions (hAMSCs after hypoxia), or treated with IFN-γ (hAMSCs after IFN-γ treatment). (B) Representative images of flow cytometry analysis for quantification of hAMSCs at step 2 for both positive (CD90, <t>CD73</t> and CD13) and negative surface markers (HLA-DR and CD45). Green represents the isotype control, and red represents stained cells. (C) Experimental plan and exosome characterization (size and concentration). DIC, differential interference contrast.
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    Image Search Results


    Brain pericytes increase glutamate uptake in astrocyte cultures. (A) Double immunofluorescence staining for CD13/EAAT1 (top left panel), CD13/EAAT2 (top right panel), GFAP/EAAT1 (bottom left panel), and GFAP/EAAT2 (bottom right panel) in intact mouse hippocampus sections. CD13 and GFAP signals appear green, while EAAT1 and EAAT2 signals appear red. (B) Regression analyses of cell/medium ratios of [ 3 H]-L-glutamate ([ 3 H]-L-Glu) uptake versus incubation time in astrocytes cultured without pericytes (astrocyte monocultures) or with pericytes (pericyte co-cultures). Astrocytes in monocultures and pericyte co-cultures were incubated in uptake buffer containing [ 3 H]-L-Glu for 1, 2, 5, and 10 min. A linear regression analysis was also performed to obtain the rate of [ 3 H]-L-Glu uptake. The slope of the lines was 9.48 in astrocyte monocultures ( r 2 = 0.90) and 13.54 in pericyte co-cultures ( r 2 = 0.89). Values are expressed as means ± SEM (n = 4 per point). * p < 0.05, between astrocyte monocultures and pericyte co-cultures.

    Journal: IBRO Neuroscience Reports

    Article Title: Brain pericytes upregulate glutamate uptake by astrocytes in vitro through sodium-dependent transporter

    doi: 10.1016/j.ibneur.2025.05.017

    Figure Lengend Snippet: Brain pericytes increase glutamate uptake in astrocyte cultures. (A) Double immunofluorescence staining for CD13/EAAT1 (top left panel), CD13/EAAT2 (top right panel), GFAP/EAAT1 (bottom left panel), and GFAP/EAAT2 (bottom right panel) in intact mouse hippocampus sections. CD13 and GFAP signals appear green, while EAAT1 and EAAT2 signals appear red. (B) Regression analyses of cell/medium ratios of [ 3 H]-L-glutamate ([ 3 H]-L-Glu) uptake versus incubation time in astrocytes cultured without pericytes (astrocyte monocultures) or with pericytes (pericyte co-cultures). Astrocytes in monocultures and pericyte co-cultures were incubated in uptake buffer containing [ 3 H]-L-Glu for 1, 2, 5, and 10 min. A linear regression analysis was also performed to obtain the rate of [ 3 H]-L-Glu uptake. The slope of the lines was 9.48 in astrocyte monocultures ( r 2 = 0.90) and 13.54 in pericyte co-cultures ( r 2 = 0.89). Values are expressed as means ± SEM (n = 4 per point). * p < 0.05, between astrocyte monocultures and pericyte co-cultures.

    Article Snippet: Antibodies against CD13 were obtained from Acris Antibodies (#SM2298P; Rockville, MD, USA).

    Techniques: Double Immunofluorescence Staining, Incubation, Cell Culture

    Human amnion-derived mesenchymal stromal/stem cells (hAMSCs) were grown as monolayers with or without priming. (A) Representative DIC images of hAMSCs grown as monolayers without priming (hAMSCs), cultured under hypoxic conditions (hAMSCs after hypoxia), or treated with IFN-γ (hAMSCs after IFN-γ treatment). (B) Representative images of flow cytometry analysis for quantification of hAMSCs at step 2 for both positive (CD90, CD73 and CD13) and negative surface markers (HLA-DR and CD45). Green represents the isotype control, and red represents stained cells. (C) Experimental plan and exosome characterization (size and concentration). DIC, differential interference contrast.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Proteomic analysis and functional validation reveal distinct therapeutic capabilities related to priming of mesenchymal stromal/stem cells with IFN-γ and hypoxia: potential implications for their clinical use

    doi: 10.3389/fcell.2024.1385712

    Figure Lengend Snippet: Human amnion-derived mesenchymal stromal/stem cells (hAMSCs) were grown as monolayers with or without priming. (A) Representative DIC images of hAMSCs grown as monolayers without priming (hAMSCs), cultured under hypoxic conditions (hAMSCs after hypoxia), or treated with IFN-γ (hAMSCs after IFN-γ treatment). (B) Representative images of flow cytometry analysis for quantification of hAMSCs at step 2 for both positive (CD90, CD73 and CD13) and negative surface markers (HLA-DR and CD45). Green represents the isotype control, and red represents stained cells. (C) Experimental plan and exosome characterization (size and concentration). DIC, differential interference contrast.

    Article Snippet: For phenotypic characterization, hAMSCs were first washed twice with FACS buffer (PBS containing 0.3% BSA and 0.1% NaN 3 ) and then incubated on ice for 30 min with specific antibodies against CD90, CD73, CD13, CD45, and HLA-DR (BD Biosciences, United States).

    Techniques: Derivative Assay, Cell Culture, Flow Cytometry, Staining, Concentration Assay